草業(yè)科學(xué)-葉綠素?zé)晒獬上裣到y(tǒng)Weed-PAM
基于功能強大的IMAG-PAM,改進升級后適用于野外雜草,草坪等光合作用研究
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主要功能
l 成像功能:對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等至少17種參數(shù)進行成像分析。測定調(diào)節(jié)性能量耗散Y(NPQ),反映植物光保護能力,測定非調(diào)節(jié)性能量耗散Y(NO),反映植物光損傷程度。
l 程序測量功能:可程序測量熒光誘導(dǎo)曲線、快速光曲線和暗弛豫,也可手動測量;在測量過程中能自動分析所有熒光參數(shù)的變化趨勢
l AOI功能:可在測量前或測量后任意選擇感興趣的區(qū)域(AOI),程序?qū)⒆詣訉x擇的AOI的數(shù)據(jù)進行變化趨勢分析,并在報告文件中顯示相關(guān)AOI的數(shù)據(jù)。所有報告文件中顯示的數(shù)據(jù)都可導(dǎo)出到EXCEL文件中。
l 成像異質(zhì)性分析功能:對任意參數(shù)任意時間的成像,可在圖像上任意選取兩點,軟件自動對兩點間的數(shù)據(jù)進行橫向異質(zhì)性分析,并可導(dǎo)出到EXCEL文件中。
l 成像數(shù)據(jù)范圍分析功能:對任意參數(shù)任意時間的成像,可分析任意兩個熒光數(shù)值之間有多少個像素點,多少面積(cm2)。
l 突變株篩選功能:可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆(抗鹽、抗旱、抗病等)等突變株。
l 吸光系數(shù)測量功能:快速測量葉片的吸光系數(shù)。吸光系數(shù)測量光源: 16個紅光(650 nm)和16個近紅外(780 nm)LED,用于測量植物葉片或藻類樣品PAR吸光系數(shù)。
成像參數(shù)
l Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等
技術(shù)參數(shù)
Maxi探頭(藍光版)藍色LED,450 nm
Maxi探頭(紅光版)紅色LED,650 nm
Mini探頭(藍光版)藍色LED,460 nm
Mini探頭(紅光版)紅色LED,620 nm
應(yīng)用領(lǐng)域
l 光合作用研究:可以在相同的條件下同時對大量樣品進行成像
l 植物病理學(xué):病斑部位(包括肉眼不可見時)成像以及病斑擴散的時空動力學(xué)
l 植物脅迫生 理學(xué):肉眼不可見脅迫損傷的早期檢測
l 遺傳育種:出苗后大規(guī)??焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株
l 突變株篩選:快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等
l 分子生物學(xué):宏觀水平上檢測樣品的綠色熒光蛋白(GFP)熒光
l 其它多種擴展研究
參考文獻
數(shù)據(jù)來源:光合作用文獻 Endnote 數(shù)據(jù)庫,更新至 2021 年 1 月,文獻數(shù)量超過 10000 篇
原始數(shù)據(jù)來源:Google Scholar
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